Blood Draw Ucsf Mission Bay Hours
Overview of Services
Management
Kirsten Mowry, PA (ASCP)cm - Interim Tissue Core Manager (kirsten.mowry@ucsf.edu)
Location and hours of operation
| Hours | Locations |
| 8:00am - 4:30 pm Monday - Friday (excluding UCSF holidays) | Mission Bay: 1825 4th St., M2391 Parnassus: 513 Parnassus Ave, S526 Mt. Zion: 2340 Sutter St., S241 |
Links and Resources
- Team website: Biospecimen Resources Program
- Team email: biobank@ucsf.edu
- Team messaging: ucsfbiobank.slack.com
- UCSF Core Services and Equipment Search
Search available services:
View: by category alphabetically
| Name | Description | Price |
|---|---|---|
| Aliquoting Only | Transfer of unprocessed blood or body fluids into storage tubes (cryovials etc). No other processing. | Inquire |
| Biopsy Collection | A biobank technician attends biopsy procedure and coordinates with research staff and clinical staff to collect specified type and quantiy of material required for study. Tissue is collected and preserved in the procedure room to ensure viability. | Inquire |
| Blood Processing | Centrifugation of blood collection tubes to isolate serum, plasma or buffy coat. Pooled blood tubes are treated as a single specimen; each additional tube type or special processing step is subject to additional fee. | Inquire |
| Body Fluid Processing | Centrifuge, wash and pellet up to 1 L fluid. Isolate pellet and process to frozen or FFPE block. | Inquire |
| PBMC Processing | Isolation of PBMC by one of the Biobank Laboratory standard protocols.
| Inquire |
| RNA or DNA Extraction | The Biobank & Processing Core extracts DNA from formalin-fixed paraffin embedded (FFPE) tissue, frozen tissue blood and body fluids. Our protocols utilize commercially available kits and methods have been further optimized in house. DNA & RNA quantitation via Nanodrop. Sample preparation, specialty kits, small runs and custom methods are subject to additional fees. Frozen Tissue:Genotyping and other DNA analyses using PCR or next-generation sequencing (NGS) require a suitable amount of high-quality genomic DNA as starting material. The most common bottleneck to obtaining high-quality DNA is tissue lysis. Fibrous or other hard-to-lyse tissues require lengthy and tedious protocols that often result in low yields of low-quality DNA. The QIAamp Fast DNA Tissue Kit overcomes this bottleneck by providing innovative disruption tubes that significantly improve yield and shorten the overall time to result. Blood & Body Fluids: Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.
Formalin Fixed Paraffin Embedded Tissue*: Artificial C>T mutations caused by cytosine deamination are critical in next-generation sequencing (NGS). These artifacts are caused by formalin fixation and aging, and result in sequencing errors. The GeneRead FFPE purification procedure also includes enzymatic removal of these artifacts, while ensuring high yields and purity, making it especially suitable for NGS applications. PAXgene Blood DNA*:Purification of up to 500 µg genomic DNA from fresh or frozen whole blood collected. Used in conjunction with PAXgene DNA blood collection tubes. PAXgene Blood RNA*:Purification of intracellular RNA from whole blood for applications in molecular diagnostic testing, such as real-time RT-PCR analysis of specific transcripts. Used in conjunction with PAXGene RNA blood collection tubes. * Subject to specialty kit surcharge | Inquire |
| Tissue Preparation Sets | Tissue preparation* service sets:
*All reagents and supplies are provided by Biobank. | Inquire |
| Urine Processing | Centrifugation of urine with aliquoting of supernate and cell pellet isolation. Up to 100mL and 10 aliquots. | Inquire |
| ▼ ► Biopsy (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| Biopsy Collection | A biobank technician attends biopsy procedure and coordinates with research staff and clinical staff to collect specified type and quantiy of material required for study. Tissue is collected and preserved in the procedure room to ensure viability. | Inquire | |
| ▼ ► Services (6) | |||
| Name | Description | Price | |
| Blood Processing | Centrifugation of blood collection tubes to isolate serum, plasma or buffy coat. Pooled blood tubes are treated as a single specimen; each additional tube type or special processing step is subject to additional fee. | Inquire | |
| PBMC Processing | Isolation of PBMC by one of the Biobank Laboratory standard protocols.
| Inquire | |
| Aliquoting Only | Transfer of unprocessed blood or body fluids into storage tubes (cryovials etc). No other processing. | Inquire | |
| Urine Processing | Centrifugation of urine with aliquoting of supernate and cell pellet isolation. Up to 100mL and 10 aliquots. | Inquire | |
| Body Fluid Processing | Centrifuge, wash and pellet up to 1 L fluid. Isolate pellet and process to frozen or FFPE block. | Inquire | |
| RNA or DNA Extraction | The Biobank & Processing Core extracts DNA from formalin-fixed paraffin embedded (FFPE) tissue, frozen tissue blood and body fluids. Our protocols utilize commercially available kits and methods have been further optimized in house. DNA & RNA quantitation via Nanodrop. Sample preparation, specialty kits, small runs and custom methods are subject to additional fees. Frozen Tissue:Genotyping and other DNA analyses using PCR or next-generation sequencing (NGS) require a suitable amount of high-quality genomic DNA as starting material. The most common bottleneck to obtaining high-quality DNA is tissue lysis. Fibrous or other hard-to-lyse tissues require lengthy and tedious protocols that often result in low yields of low-quality DNA. The QIAamp Fast DNA Tissue Kit overcomes this bottleneck by providing innovative disruption tubes that significantly improve yield and shorten the overall time to result. Blood & Body Fluids: Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.
Formalin Fixed Paraffin Embedded Tissue*: Artificial C>T mutations caused by cytosine deamination are critical in next-generation sequencing (NGS). These artifacts are caused by formalin fixation and aging, and result in sequencing errors. The GeneRead FFPE purification procedure also includes enzymatic removal of these artifacts, while ensuring high yields and purity, making it especially suitable for NGS applications. PAXgene Blood DNA*:Purification of up to 500 µg genomic DNA from fresh or frozen whole blood collected. Used in conjunction with PAXgene DNA blood collection tubes. PAXgene Blood RNA*:Purification of intracellular RNA from whole blood for applications in molecular diagnostic testing, such as real-time RT-PCR analysis of specific transcripts. Used in conjunction with PAXGene RNA blood collection tubes. * Subject to specialty kit surcharge | Inquire | |
| ▼ ► Surgical Collection (1) | |||
| Name | Description | Price | |
| Tissue Preparation Sets | Tissue preparation* service sets:
*All reagents and supplies are provided by Biobank. | Inquire | |
Blood Draw Ucsf Mission Bay Hours
Source: https://ucsf.ilab.agilent.com/service_center/show_external/5250?name=biobank-sample-processing-core
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