Blood Draw Ucsf Mission Bay Hours

Overview of Services

Management

Kirsten Mowry, PA (ASCP)cm - Interim Tissue Core Manager (kirsten.mowry@ucsf.edu)

Location and hours of operation

Hours Locations

8:00am - 4:30 pm

Monday - Friday

(excluding UCSF holidays)

Mission Bay: 1825 4th St., M2391

Parnassus: 513 Parnassus Ave, S526

Mt. Zion:  2340 Sutter St., S241

Links and Resources

  1. Team website: Biospecimen Resources Program
  2. Team email: biobank@ucsf.edu
  3. Team messaging: ucsfbiobank.slack.com
  4. UCSF Core Services and Equipment Search

Search available services: spinner View: by category alphabetically

Name Description Price
Aliquoting Only

Transfer of unprocessed blood or body fluids into storage tubes (cryovials etc). No other processing.

Inquire
Biopsy Collection

A biobank technician attends biopsy procedure and coordinates with research staff and clinical staff to collect specified type and quantiy of material required for study.  Tissue is collected and preserved in the procedure room to ensure viability.

Inquire
Blood Processing

Centrifugation of blood collection tubes to isolate serum, plasma or buffy coat. Pooled blood tubes are treated as a single specimen; each additional tube type or special processing step is subject to additional fee.

Inquire
Body Fluid Processing

Centrifuge, wash and pellet up to 1 L fluid.  Isolate pellet and process to frozen or FFPE block.

Inquire
PBMC Processing

Isolation of PBMC by one of the Biobank Laboratory standard protocols.

  • SepMate Isolation Method (Ficoll)
  • CPT Tube Processing
Inquire
RNA or DNA Extraction

The Biobank & Processing Core extracts DNA from formalin-fixed paraffin embedded (FFPE) tissue, frozen tissue blood and body fluids.  Our protocols utilize commercially available kits and methods have been further optimized in house. DNA & RNA quantitation via Nanodrop. Sample preparation, specialty kits, small runs and custom methods are subject to additional fees.

Frozen Tissue:Genotyping and other DNA analyses using PCR or next-generation sequencing (NGS) require a suitable amount of high-quality genomic DNA as starting material. The most common bottleneck to obtaining high-quality DNA is tissue lysis. Fibrous or other hard-to-lyse tissues require lengthy and tedious protocols that often result in low yields of low-quality DNA. The QIAamp Fast DNA Tissue Kit overcomes this bottleneck by providing innovative disruption tubes that significantly improve yield and shorten the overall time to result.

Blood & Body Fluids:  Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.

    • Fresh and frozen whole blood or buffy coat
    • Plasma or serum
    • Bone marrow
    • Lymphocytes
    • Platelets
    • Body fluids

Formalin Fixed Paraffin Embedded Tissue*: Artificial C>T mutations caused by cytosine deamination are critical in next-generation sequencing (NGS). These artifacts are caused by formalin fixation and aging, and result in sequencing errors. The GeneRead FFPE purification procedure also includes enzymatic removal of these artifacts, while ensuring high yields and purity, making it especially suitable for NGS applications.

PAXgene Blood DNA*:Purification of up to 500 µg genomic DNA from fresh or frozen whole blood collected.   Used in conjunction with PAXgene DNA blood collection tubes.

PAXgene Blood RNA*:Purification of intracellular RNA from whole blood for applications in molecular diagnostic testing, such as real-time RT-PCR analysis of specific transcripts. Used in conjunction with PAXGene RNA blood collection tubes.

* Subject to specialty kit surcharge

Inquire
Tissue Preparation Sets

Tissue preparation* service sets:

  • Snap frozen in cryovials (5 vials)
  • OCT Embedded and frozen in molds (2 molds)
  • Viable freeze with cell preservation media (2 vials)
  • RNAlater treated and frozen (2 vials)
  • Fresh tissue in culture media (1 tube)

*All reagents and supplies are provided by Biobank.

Inquire
Urine Processing

Centrifugation of urine with aliquoting of supernate and cell pellet isolation.  Up to 100mL and 10 aliquots.

Inquire
Biopsy (1)
Name Description Price
Biopsy Collection

A biobank technician attends biopsy procedure and coordinates with research staff and clinical staff to collect specified type and quantiy of material required for study.  Tissue is collected and preserved in the procedure room to ensure viability.

Inquire
Services (6)
Name Description Price
Blood Processing

Centrifugation of blood collection tubes to isolate serum, plasma or buffy coat. Pooled blood tubes are treated as a single specimen; each additional tube type or special processing step is subject to additional fee.

Inquire
PBMC Processing

Isolation of PBMC by one of the Biobank Laboratory standard protocols.

  • SepMate Isolation Method (Ficoll)
  • CPT Tube Processing
Inquire
Aliquoting Only

Transfer of unprocessed blood or body fluids into storage tubes (cryovials etc). No other processing.

Inquire
Urine Processing

Centrifugation of urine with aliquoting of supernate and cell pellet isolation.  Up to 100mL and 10 aliquots.

Inquire
Body Fluid Processing

Centrifuge, wash and pellet up to 1 L fluid.  Isolate pellet and process to frozen or FFPE block.

Inquire
RNA or DNA Extraction

The Biobank & Processing Core extracts DNA from formalin-fixed paraffin embedded (FFPE) tissue, frozen tissue blood and body fluids.  Our protocols utilize commercially available kits and methods have been further optimized in house. DNA & RNA quantitation via Nanodrop. Sample preparation, specialty kits, small runs and custom methods are subject to additional fees.

Frozen Tissue:Genotyping and other DNA analyses using PCR or next-generation sequencing (NGS) require a suitable amount of high-quality genomic DNA as starting material. The most common bottleneck to obtaining high-quality DNA is tissue lysis. Fibrous or other hard-to-lyse tissues require lengthy and tedious protocols that often result in low yields of low-quality DNA. The QIAamp Fast DNA Tissue Kit overcomes this bottleneck by providing innovative disruption tubes that significantly improve yield and shorten the overall time to result.

Blood & Body Fluids:  Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.

    • Fresh and frozen whole blood or buffy coat
    • Plasma or serum
    • Bone marrow
    • Lymphocytes
    • Platelets
    • Body fluids

Formalin Fixed Paraffin Embedded Tissue*: Artificial C>T mutations caused by cytosine deamination are critical in next-generation sequencing (NGS). These artifacts are caused by formalin fixation and aging, and result in sequencing errors. The GeneRead FFPE purification procedure also includes enzymatic removal of these artifacts, while ensuring high yields and purity, making it especially suitable for NGS applications.

PAXgene Blood DNA*:Purification of up to 500 µg genomic DNA from fresh or frozen whole blood collected.   Used in conjunction with PAXgene DNA blood collection tubes.

PAXgene Blood RNA*:Purification of intracellular RNA from whole blood for applications in molecular diagnostic testing, such as real-time RT-PCR analysis of specific transcripts. Used in conjunction with PAXGene RNA blood collection tubes.

* Subject to specialty kit surcharge

Inquire
Surgical Collection (1)
Name Description Price
Tissue Preparation Sets

Tissue preparation* service sets:

  • Snap frozen in cryovials (5 vials)
  • OCT Embedded and frozen in molds (2 molds)
  • Viable freeze with cell preservation media (2 vials)
  • RNAlater treated and frozen (2 vials)
  • Fresh tissue in culture media (1 tube)

*All reagents and supplies are provided by Biobank.

Inquire

Blood Draw Ucsf Mission Bay Hours

Source: https://ucsf.ilab.agilent.com/service_center/show_external/5250?name=biobank-sample-processing-core

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